This light microscopy image shows keratin tonofilaments (red) linking the cell sheet through connecting desmosomes, which are highlighted by desmoplakin (green). Published in Green KJ and Gaudry CA. Are desmosomes more than tethers for intermediate filaments. Nat Rev Mol Cell Biol 1:208, 2000.This light microscopy image shows keratin tonofilaments (red) linking the cell sheet through connecting desmosomes, which are highlighted by desmoplakin (green). Published in Green KJ and Gaudry CA. Are desmosomes more than tethers for intermediate filaments. Nat Rev Mol Cell Biol 1:208, 2000.
Immunofluorescence analysis of human keratinocytes that have been silenced for the desmosome constituent and armadillo protein family member, plakophilin 2. The focal adhesion marker, zyxin, was used to illustrate the presence of many large and mature focal adhesions that are observed upon loss of plakophilin 2.Immunofluorescence analysis of human keratinocytes that have been silenced for the desmosome constituent and armadillo protein family member, plakophilin 2. The focal adhesion marker, zyxin, was used to illustrate the presence of many large and mature focal adhesions that are observed upon loss of plakophilin 2.
Colony of stratifying cells stained for f-actin using fluorescently-conjugated phalloidin (red), DAPI to stain DNA (blue) and a cytoplasmic protein (green).Colony of stratifying cells stained for f-actin using fluorescently-conjugated phalloidin (red), DAPI to stain DNA (blue) and a cytoplasmic protein (green).
A transmission electron micrograph of two adjacent keratinocytes illustrating the spatial organization of the cell-cell junctions, desmosomes and adherens junctions, along the membrane.A transmission electron micrograph of two adjacent keratinocytes illustrating the spatial organization of the cell-cell junctions, desmosomes and adherens junctions, along the membrane.
Colony of stratifying epithelial cells with differentiating cells in green sitting on top of a basal layer in red from which they have emerged.Colony of stratifying epithelial cells with differentiating cells in green sitting on top of a basal layer in red from which they have emerged.
An image of an organotypic model of human epidermis typically employed in the lab to study skin development and homeostasis. This 3D epidermal “raft” is produced by plating freshly isolated primary human keratinocytes on a collagen lattice plug grown on an air liquid interface. These skin equivalents allow for the analysis of differentiation in a physiologically relevant system.An image of an organotypic model of human epidermis typically employed in the lab to study skin development and homeostasis. This 3D epidermal “raft” is produced by plating freshly isolated primary human keratinocytes on a collagen lattice plug grown on an air liquid interface. These skin equivalents allow for the analysis of differentiation in a physiologically relevant system.
Cells grown on patterns that have been coated with extracellular matrix. Green shows points of contact between cells and the substrate, while the actin cytoskeleton is labeled with fluorescently conjugated phalloidin and shown in red.Cells grown on patterns that have been coated with extracellular matrix. Green shows points of contact between cells and the substrate, while the actin cytoskeleton is labeled with fluorescently conjugated phalloidin and shown in red.
An electron micrograph of a bovine tongue desmosome. Published in Yin T, Green KJ: Regulation of desmosome assembly and adhesion. Semin Cell Develop Biol 15:665, 2004.An electron micrograph of a bovine tongue desmosome. Published in Yin T, Green KJ: Regulation of desmosome assembly and adhesion. Semin Cell Develop Biol 15:665, 2004.
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Just as communication between people is essential for our society to thrive, so too is communication between cells in a multicellular organism essential for its existence. Our goal is to understand the molecular basis for how cells stick together, not only to provide mechanical strength to tissues, but also to regulate chemical signals important for development and differentiation. We are particularly interested in tissues such as skin and heart that are major targets for adhesion-related diseases, including inherited, autoimmune and bacterial-toxin mediated disorders and cancer. The Green laboratory provides an open, collaborative, congenial research environment promoting high impact research while mentoring students and fellows for a future as independent scientists and educators. Enjoy our site, and please contact any one of us if you need further information.
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