Resources

The Burridge Lab is dedicated to the elimination of the use of animals and animal-derived products in our research.

FBS: We have eliminated FBS from the lab and replaced it with Cosmic Calf Serum, which was the best performing FBS alternative in our hands. We are actively trying to fully eliminate CCS from all of our prototcols.

Albumin: We are actively progressing towards the development of fully albumin-free cell culture protocols with lower cost than albumin-containing protocols.

Matrigel: We have found that Matrigel can be replaced with Synthemax with only minor tweeks to differentation protocols. Cost is similar.

Antibodies: We can not longer recommend recombinant antibodies due to the substantial increases in price. We recommend searching DSHB (below) or finding reasonably priced monoclonal antibodies.

Secondary antibodies: We are currently testing Proteintech's Multi-rAb recombinant monoclonal secondary antibodies.

Staining: For both flow cytometry and immunofluoresecent staining we are assessing Vector Labs Animal-Free Blocker.

The Burridge Lab is a proponent of open-access resources.

We endeavor to publish all of our pre-print manuscripts on BioRxiv. All of our published papers are available below.

All of the plasmids included in our publications are available from Addgene.

Where possible we use antibodies provided by the Developmental Studies Hybridoma Bank.

Some of our favorites include: SSEA4, POU5F1, NANOG, SOX2, TNNT2, TNNI3, ACTN1.

RNA-seq data for our published projects are available from GEO.

Resources

PDFs of Recent Publications