Protocols & Reagents
Validated Antibodies for IHC
Validated Antibodies for Western blots
Secondary Antibodies
Antibodies being tested
Angiogenesis Assays
Animal
- Aorta EdU Staining
- Cutaneous Wound Healing Assay
- EC Isolation Modif.
- En Face Aorta Staining
- FACS Staining Protocol
- Modified Matrigel Assay
- Mouse Bone Marrow Transplantation
- Ovarian Transplant
- Superovulation
- Tamoxifen Protocol
- Vascular Permeability
- Whole-Mt ISH_Chick or Mouse Embryo
- Xenograft TumorAssay
- X-Gal staining for expression of LacZ
Biochemistry
- Biotinylated VEGF digestion
- Immunoprecipitation
- LacZ Staining
- Membrane Protein labeling
- Membrane Stripping
- Silver Staining
- Tricine Gel after VEGF digestion
- VEGF Western on Tricine Gel
- Zymogram Protocol
Genotyping
Immunohistochemistry
- Citrate Buffer Epitope Retrieval
- FACS Staining Protocol
- Hamster AntiMouse CD31
- IHC - CD31 Paraffin Sections
- IHC Whole Mt Tissue PECAM
- LacZ Staining
- MLC for Cells in Suspension
- Modified LacZ staining
- PECAM Protocol
- QH-1 Antibody Staining - Quail
- VE-Cadherin Protocol
- Whole Mt Retina IHC
Molecular Biology
- Annealing of Adapters
- Blue White Color Screening
- Genotyping TGF-alpha Transgenic mice
- Intima Isolation from Aorta for scRNAseq
- Ligations - Vector Insert Ratio
- Miniprep DNA
- Nanostring Counter Sample Collection SOP
- Realtime PCR Protocol
- RNA Extraction from Cells
- RNA Extraction Solutions
- RNA Gel
- RNA Protection Assay
- Whole Mount In Situ Hybridization Protocol
Protein Chemistry
- 3D Vitrogen Gel
- ADAMTS1 Purification
- Adhesion Assays
- BAEC Isolation
- Cell Surface Biotinylation
- Chrystal Violet Cell Staining & Clone Counting
- Coating VEGF Bound Plate
- Immunoprecipitation
- Iodination Injection of 125I-Protein
- Isolation of HDEC Isolation
- Isolation of Ovarian Epithelial Cells
- Labeling Cells with Live Dyes
- Soft Agar Colony Formation
- Transfection of T47D Cells
Subcloning Guide
Tissue Culture
- 35S-methionine Labeling
- CaPO4 Transfection
- Immunocytochemistry
- Integrin Receptor Ligand Binding Assays
- Isolation of EC from Embryo
- Isolation of ECs from Mouse Embryos
- Isolating BAEC
- KDR Phosphorylation Assay
- Lentiviral Infection of ECs (HDMEC)
- MAEC isolation
- Media Recipes
- Polyoma Middle T Production (GgP+E Cell Line)
- Staining Protocol for FACS
- X-Gal Staining for LacZ Expression
Vascular Imaging
- DAB Immuno-staining for CD-31
- ICC Protocol
- IHC Protocol for Paraffin-embedded sections - PECAM Antibody staining
- IHC Protocol for Whole Mt - Notch Antibody staining
- IHC Protocol for Whole-Mt - PECAM Antibody staining
- Immuno-staining for CD-31
- Immunoperoxidase of Paraffin-embedded sections
- Labeling Cells with Live Dyes
- Vibratome Protocol
- Whole-mount Lectin Staining using Vector Elite ABC Kit
Our laboratory has developed a constitutive VE Cre-Recombinase mouse line available to the scientific community through the Jax labs.
Constitutive VE-Cadherin Cre-Recombinase
Inducible VE-Cadherin Cre-Recombinase
Floxed Progesterone Receptor
- Publication characterizing the model
- PRCE Genotyping Protocol
- Resulting publications using Floxed Progest