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Publications

Original Investigations

2024

The hexosamine biosynthetic pathway rescues lysosomal dysfunction in Parkinson's disease patient iPSC derived midbrain neurons. Wani WY, Zunke F, Belur NR, Mazzulli JR. Nat Commun. 2024 Jun 19;15(1):5206. doi: 10.1038/s41467-024-49256-3. PMID: 38897986

Nuclear aggregates of NONO/SFPQ and A-to-I-edited RNA in Parkinson's disease and dementia with Lewy bodies.Belur NR, Bustos BI, Lubbe SJ, Mazzulli JR. Neuron. 2024 Aug 7;112(15):2558-2580.e13. doi: 10.1016/j.neuron.2024.05.003. Epub 2024 May 17. PMID: 38761794

α-Synuclein: Multiple pathogenic roles in trafficking and proteostasis pathways in Parkinson's disease. Zalon AJ, Quiriconi DJ, Pitcairn C, Mazzulli JR. Neuroscientist. 2024 Feb 29:10738584241232963. doi: 10.1177/10738584241232963. Online ahead of print. PMID: 38420922

Death Induced by Survival gene Elimination (DISE) correlates with neurotoxicity in Alzheimer's disease and aging. Paudel B, Jeong SY, Martinez CP, Rickman A, Haluck-Kangas A, Bartom ET, Fredriksen K, Affaneh A, Kessler JA, Mazzulli JR, Murmann AE, Rogalski E, Geula C, Ferreira A, Heckmann BL, Green DR, Sadleir KR, Vassar R, Peter ME. Nat Commun. 2024 Jan 18;15(1):264. doi: 10.1038/s41467-023-44465-8. PMID: 38238311

2023

Pitcairn C, Murata N, Zalon A, Stojkovska I, Mazzulli JR. Impaired autophagic-lysosomal fusion in Parkinson's patient midbrain neurons occurs through loss of ykt6 and is rescued by farnesyltransferase inhibition. J. Neurosci, 2023 Feb 13:JN-RM-0610-22. doi: 10.1523/JNEUROSCI.0610-22.2023, PMID: 36788031

Drobny A, Boros FA, Balta D, Prieto Huarcaya S, Caylioglu D, Qazi N, Vandrey J, Schneider Y, Dobert JP, Pitcairn C, Mazzulli JR, Zunke F. Reciprocal effects of alpha-synuclein aggregation and lysosomal homeostasis in synucleinopathy models. Transl Neurodegener. 2023 Jun 13;12(1):31. doi: 10.1186/s40035-023-00363-z. PMID: 37312133

Fagen SJ, Burgess JD, Lim MJ, Amerna D, Kaya ZB, Faroqi AH, Perisetla P, DeMeo NN, Stojkovska I, Quiriconi DJ, Mazzulli JR, Delenclos M, Boschen SL, McLean PJ. Honokiol decreases alpha-synuclein mRNA levels and reveals novel targets for modulating alpha-synuclein expression. Front Aging Neurosci. 2023 Aug 10;15:1179086. doi: 10.3389/fnagi.2023.1179086. eCollection 2023. PMID: 37637959

2022

Stojkovska I, Wani WY, Zunke F, Belur NR, Pavlenko EA, Mwenda N, Sharma K, Francelle L, Mazzulli JR. Rescue of α-synuclein aggregation in Parkinson’s patient neurons by synergistic enhancement of ER proteostasis and protein trafficking. Neuron, Feb 2, 2022, PMID: 34793693

Chen X, Dong T, Hu Y, Shaffo FC, Belur NR, Mazzulli JR, Gray SJ. AAV9/MFSD8 gene therapy is effective in preclinical models of neuronal ceroid lipofuscinosis type 7. J. Clin Invest. 2022 Jan 13:e146286. doi: 10.1172/JCI146286. PMID: 35025759

Francelle L, Mazzulli JR. Neuroinflammation in Gaucher Disease, neuronal ceroid lipofuscinosis, and commonalities with Parkinson's disease. Brain Res. 2022 Jan 19;1780:147798. doi: 10.1016/j.brainres.2022.147798. PMID: 35063468

Heinl ES, Lorenz S, Schmidt B, Nasser M Laqtom N, Mazzulli JR, Francelle L, Yu TW, Greenberg B, Storch S, Tegtmeier I, Othmen H, Maurer K, Steinfurth M, Witzgall R, Milenkovic V, Wetzel CH, Reichold M. CLN7/MFSD8 may be an important factor for SARS-CoV-2 cell entry. iScience, 2022 Oct 21;25(10):105082. doi: 10.1016/j.isci.2022.105082. PMID: 36093380

Cuddy LK, Alia AO, Salvo MA, Chandra S, Grammatopoulos TN, Justman CJ, Lansbury PT Jr, Mazzulli JR, Vassar R. Farnesyltransferase inhibitor LNK-754 attenuates axonal dystrophy and reduces amyloid pathology in mice. Mol Neurodegener. 2022 Aug 20;17(1):54. doi: 10.1186/s13024-022-00561-9. PMID: 35987691

Prieto Huarcaya S, Drobny A, Marques ARA, Di Spiezio A, Dobert JP, Balta D, Werner C, Rizo T, Gallwitz L, Bub S, Stojkovska I, Belur NR, Fogh J, Mazzulli JR, Xiang W, Fulzele A, Dejung M, Sauer M, Winner B, Rose-John S, Arnold P, Saftig P, Zunke F. Recombinant pro-CTSD (cathepsin D) enhances SNCA/α-Synuclein degradation in α-Synucleinopathy models. Autophagy. 2022 May;18(5):1127-1151. doi: 10.1080/15548627.2022.2045534. Epub 2022 Apr 28. PMID: 35287553

2021

Fredriksen, K.Aivazidis S, Sharma K, Burbidge KJ, Pitcairn C, Zunke F, Gelyana E, Mazzulli JR. Pathological a-syn aggregation is mediated by glycosphingolipid chain length and the physiological state of a-synuclein in vivo. Proc Natl Acad Sci U S A Dec 14th, 2021. PMID: 34893541

Cuddy LK, Mazzulli JR. Analysis of lysosomal hydrolase trafficking and activity in human iPSC-derived neuronal models. STAR Protoc. 2021 Feb 13;2(1):100340.  doi: 10.1016/j.xpro.2021.100340. eCollection 2021 Mar 19. PMID: 33659904

Stojkovska I, Mazzulli JR. Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue. STAR Protoc. 2021 Mar 6;2(1):100372.  doi: 10.1016/j.xpro.2021.100372.  eCollection 2021 Mar 19. PMID: 33733241

2019

Cuddy LK, Wani WY, Morella ML, Pitcairn C, Tsutsumi K, Fredriksen K, Justman CJ, Grammatopoulos TN, Belur NR, Zunke F, Subramanian A, Affaneh A, Lansbury PT Jr, Mazzulli JR. Stress-Induced Cellular Clearance Is Mediated by the SNARE Protein ykt6 and Disrupted by α-Synuclein. Neuron. 2019 Oct 21. pii: S0896-6273(19)30774-3. doi: 10.1016/j.neuron.2019.09.001. [Epub ahead of print]. PMID: 31648898

Kim J, Hu C, Moufawad El Achkar C, Black LE, Douville J, Larson A, Pendergast MK, Goldkind SF, Lee EA, Kuniholm A, Soucy A, Vaze J, Belur NR, Fredriksen K, Stojkovska I,....Mazzulli JR, Bodamer O, Berde CB, Yu TW, Patient-Customized Oligonucleotide Therapy for a Rare Genetic Disease. N Engl J Med. 2019 Oct 24;381(17):1644-1652. doi: 10.1056/NEJMoa1813279. Epub 2019 Oct 9. PMID: 31597037

Zunke F, Mazzulli JR. Modeling neuronopathic storage diseases with patient-derived culture systems. Neurobiol Dis. July, 2019; 127:147-162. PMID:30790616

Tsunemi T, Perez-Rosello T, Ishiguro Y, Yoroisaka A, Jeon S, Hamada K, Rammonhan M, Wong YC, Xie Z, Akamatsu W, Mazzulli JR, Surmeier DJ, Hattori N, Krainc D. Increased Lysosomal Exocytosis Induced by Lysosomal Ca2+ Channel Agonists Protects Human Dopaminergic Neurons from α-Synuclein Toxicity. J Neurosci. July 17, 2019; 39(29):5760-5772. PMID:31097622

2018

Zunke F, Moise AC, Belur NR, Gelyana E, Stojkovska I, Dzaferbegovic H, Toker NJ, Jeon S, Fredriksen K, Mazzulli JR. Reversible Conformational Conversion of α-Synuclein into Toxic Assemblies by Glucosylceramide. Neuron, Jan 3rd, 2018, 97 (1), 92-107e10. PMID:29290548

Klein AD, Mazzulli JR. Is Parkinson's Disease a Lysosomal Disorder? Brain, May 30. doi: 10.1093/brain/awy147. PMID: 29860491

Pitcairn C, Wani WY, Mazzulli JR. Dysregulation of the autophagic-lysosomal pathway in Gaucher and Parkinson's disease.Neurobiol Dis. 2018 Mar 14. pii: S0969-9961(18)30074-3. doi: 10.1016/j.nbd.2018.03.008 PMID: 29550539

2017

Stojkovska I, Krainc D, Mazzulli JR. Molecular Mechanisms of a-Synuclein and GBA1 in Parkinson's Disease (2017) Cell Tissue Res. Oct 24, doi: 10.1007/s00441-017-2704-y [Epub ahead of print], PMID: 29064079

Burbulla LF, Song P, Mazzulli JR, Zampese E, Wong YC, Jeon S, Santos DP, Blanz J, Obermaier CD, Strojny C, Savas JN, Kiskinis E, Zhuang X, Krüger R, Surmeier DJ, Krainc D. Dopamine oxidation mediates mitochondrial and lysosomal dysfunction in Parkinson's disease (2017) Science. pii: eaam9080. doi: 10.1126/science.aam9080. [Epub ahead of print] PMID: 28882997

Mor DE, Tsika E, Mazzulli JR, Gould N, Kim H, Daniels MJ, Doshi S, Grossman JL, Tan VX, Kalb RG, Caldwell KA, Caldwell GA, Wolfe JH, Ischiropoulos H (2017). Dopamine induced nigrostriatal degeneration is mediated by soluble alpha-synuclein oligomers. Nature Neurosci. 2017 Nov;20(11):1560-1568. doi: 10.1038/nn.4641. Epub 2017 Sep 18. PMID:28920936

2016

Chung SY*, Kishinevsky S*, Mazzulli JR, Graziotto J, Mrejeru A, Mosharov EV, Puspita L, Valiulahi P, Sulzer D, Milner TA, Taldone T, Krainc D, Studer L, Shim JW. Parkin and PINK1 Patient iPSC-Derived Midbrain Dopamine Neurons Exhibit Mitochondrial Dysfunction and α-Synuclein Accumulation. * Co-First Author. Stem Cell Reports, 2016 Sep 15. pii: S2213-6711(16)30179-5. PMID: 27641647  * co-first author.

Mazzulli JR, Zunke F, Tsunemi T, Toker NJ, Jeon S, Burbulla LF, Patnaik S, Sidransky E, Marugan JJ, Sue CM, Krainc D. Activation of b-Glucocerebrosidase Reduces Pathological a-Synuclein and Restores Lysosomal Function in Parkinson's Patient Midbrain Neurons.  J Neurosci,  Jul 20, 2016;36(29):7693-706. PMID: 27445146

Mazzulli JR, Zunke F, Isacson O, Studer L, Krainc D. α-Synuclein-induced lysosomal dysfunction occurs through disruptions in protein trafficking in human midbrain synucleinopathy models.  Proceedings of the National Academy of Sciences USA, Feb 16, 2016 ;113(7):1931-6. PMID: 26839413

Mazzulli JR, Burbulla LF, Krainc D, Ischiropoulos H.  Detection of Free and Protein-Bound ortho-Quinones by Near-Infrared Fluorescence.  Analytical Chemistry, Feb 16, 88(4):2399-405, 2016, PMID:26813311

Zunke F, Andresen L, Wesseler S, Groth J, Arnold P, Rothaug M, Mazzulli JR, Krainc D, Blanz J, Saftig P, Schwake M. Characterization of the complex formed by β-glucocerebrosidase and the lysosomal integral membrane protein type-2. Proc Natl Acad Sci U S A, Mar 21, 2016, PMID: 27001828

2014

Rothaug M, Zunke F, Mazzulli JR, Schweizer M, Altmeppen H, Lüllmann-Rauch R, Kallemeijn WW, Gaspar P, Aerts JM, Glatzel M, Saftig P, Krainc D, Schwake M, Blanz J. LIMP-2 expression is critical for b-glucocerebrosidase activity and a-synuclein clearance. Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):15573-8. doi: 10.1073/pnas.1405700111. Epub 2014 Oct 14. PMID: 25316793 [PubMed - in process]

Knight AL, Yan X, Hamamichi S, Ajjuri RR, Mazzulli JR, Zhang MW, Daigle JG, Zhang S, Borom AR, Roberts LR, Lee SK, DeLeon SM, Viollet-Djelassi C, Krainc D, O'Donnell JM, Caldwell KA, Caldwell GA. The glycolytic enzyme, GPI, is a functionally conserved modifier of dopaminergic neurodegeneration in Parkinson's models. Cell Metab. 2014 Jul 1;20(1):145-57: PMID: 24882066.

2013

Chung CY*, Khurana V*, Auluck PK, Tardiff DF, Mazzulli JR, Soldner F, Baru V, Lou Y, Freyzon Y, Cho S, Mungenast AE, Muffat J, Mitalipova M, Pluth MD, Jui NT, Schüle B, Lippard SJ, Tsai LH, Krainc D, Buchwald SL, Jaenisch R, Lindquist S. Identification and rescue of α-synuclein toxicity in Parkinson patient-derived neurons. Science. 2013 Nov 22;342 (6161):983-987. PMID: 24158904. * co-first author

Schultheis PJ, Fleming SM, Clippinger AK, Lewis J, Tsunemi T, Giasson B, Dickson DW, Mazzulli JR, Bardgett ME, Haik KL, Ekhator O, Chava AK, Howard J, Gannon M, Hoffman E, Chen Y, Prasad V, Linn SC, Tamargo RJ, Westbroek W, Sidransky E, Krainc D, Shull GE. Atp13a2-Deficient Mice Exhibit Neuronal Ceroid Lipofuscinosis, Limited α-Synuclein Accumulation, and Age-Dependent Sensorimotor Deficits. Hum Mol Genet. 2013 Feb 7. 22 (10), 2067-2082. PMID: 23393156.

2012

Kumar KR, Ramirez A, Göbel A, Kresojević N, Svetel M, Lohmann K, M Sue C, Rolfs A, Mazzulli JR, Alcalay RN, Krainc D, Klein C, Kostic V, Grünewald A. Glucocerebrosidase mutations in a Serbian Parkinson's disease population. Eur J Neurol. 2012 Jul 20. 1468-1331. PMID: 22812582.

Cooper O, Seo H, Andrabi S, Guardia-Laguarta C, Graziotto J, Sundberg M, McLean JR, Carrillo-Reid L, Xie Z, Osborn T, Hargus G, Deleidi M, Lawson T, Bogetofte H, Perez-Torres E, Clark L, Moskowitz C, Mazzulli J, Chen L, Volpicelli-Daley L, Romero N, Jiang H, Uitti RJ, Huang Z, Opala G, Scarffe LA, Dawson VL, Klein C, Feng J, Ross OA, Trojanowski JQ, Lee VM, Marder K, Surmeier DJ, Wszolek ZK, Przedborski S, Krainc D, Dawson TM, Isacson O. Pharmacological rescue of mitochondrial deficits in iPSC-derived neural cells from patients with familial Parkinson's disease. Sci Transl Med. 2012 Jul 4;4(141):141ra90. PMID: 22764206.

Usenovic M, Tresse E, Mazzulli JR, Taylor JP, Krainc D. Deficiency of ATP13A2 leads to lysosomal dysfunction, alpha-synuclein accumulation, and neurotoxicity. J. Neurosci. Mar 21, 2012. 32, 4240-4246. PMID: 22442086

2011

Jeong H, Cohen DE, Cui L, Supinski A, Savas JN, Mazzulli JR, Yates JR., Bordone L, Guarente LP, Krainc D. Sirt1 mediates neuroprotection from mutant huntingtin by activation of TORC1 and CREB transcriptional pathway. Nat Med. Dec 18, 2011; 18(1), 159-165. PMID: 22179316.

Palada V, Terzić J, Mazzulli J, Bwala G, Hagenah J, Peterlin B, Hung AY, Klein C, Krainc D. Histamine N-methyltransferase Thr105Ile polymorphism is associated with Parkinson's disease. Neurobiol Aging. April 2011; 33(4), 836.e1-3. PMID: 21794955.

Cao K, Graziotto JJ, Blair CD, Mazzulli JR, Erdos MR, Krainc D, Collins FS. Rapamycin reverses cellular phenotypes and enhances mutant protein clearance in Hutchinson-Gilford progeria syndrome cells. Sci Transl Med. June 2011; 3 (89), 89ra58. PMID: 21715679.

Mazzulli JR, Xu Y, Sun Y, Knight AL, Mclean PJ, Caldwell GA, Sidransky E, Grabowski G, Krainc D. Gaucher disease glucocerebrosidase and alpha-synuclein form a bidirectional pathogenic loop in synucleinopathies. Cell. July 2011; 146, 37-52. PMID: 21700325.

Comment in:

  • Cookson MR. A feedforward loop links Gaucher and Parkinson’s diseases? Cell. July 2011. 146, 9-11. PMID: 21729776.
  • Dawson TM, Dawson VL. A lysosomal liar for a pathogenic protein pair. Sci Transl Med. July 2011. 3, 91ps28. PMID: 21753118.
  • Mueller K, Yeston J. Editor’s Choice: A Lysosomal Culprit. Science. July 29 2011. 333, 500-501.
  • Hawkins AK. Looping the link between Gaucher and Parkinson’s Disease. Clin Genet. Nov 2011. 80, 426-427. PMID: 21883165.
  • Fernaqut PO. Links between glucocerebrosidase and alpha-synuclein revealed. Mov. Disord. Oct. 2011. 26, 2177. PMID: 22319791.

2010

Tsika E, Moysidou M, Guo J, Cushman M, Gannon P, Sandaltzopoulos R, Giasson BI, Krainc D, Ischiropoulos H, and Mazzulli JR. Distinct region-specific alpha-synuclein oligomers in A53T transgenic mice: implications for neurodegeneration. J. Neurosci. 2010; 30:3409-3418. PMID: 20203200*Featured cover article “This Week in the Journal” J. Neurosci. 2010, 30 (9).

2009

Waxman EA, Mazzulli JR, Giasson B. Characterization of hydrophobic residue requirements for alpha-synuclein fibrillization. Biochemistry. 2009; 48: 9427-9436. PMID: 19722699.

Jeong H, Then F, Melia TJ, Mazzulli JR, Cui L, Savas JN, Voisine C, Tanese N, Hart AC, Yamamoto A, Krainc D. Acetylation targets mutant huntingtin to autophagosomes for degradation. Cell. 2009; 137: 60-72. PMID: 19345187.

2008

Martinez-Vicente M, Tallaczy Z, Kaushik S, Massey AC, Mazzulli J, Mosharov E, Hodara R, Fredenburg R, Wu DC, Follenzi A, Dauer W, Przedborski S, Ischiropoulos H, Lansbury PT, Sulzer D, Cuervo AM. Dopamine-modified alpha-synuclein blocks chaperone mediated autophagy. J Clin Invest. 2008, 118:777-788. PMID: 18172548.

2007

Mazzulli JR, Armakola M, Dumoulin M, Parastatidis I, Ischiropoulos H. Cellular oligomerization of alpha-synuclein is determined by the specific non-covalent interaction of oxidized catechols with a C-terminal sequence. J Biol Chem. 2007, 282:31621-31630. PMID: 17785456.

2006

Mazzulli JR, Mishizen AJ, Giasson BI, Lynch DR, Thomas SA, Nakashima A, Nagatsu T, Ota A, Ischiropoulos H. Cytosolic catechols inhibit alpha-synuclein aggregation and facilitate the formation of intracellular soluble oligomeric intermediates*. J Neurosci. 2006; 26:10068-10078. PMID: 17005870*Featured cover article “This Week in the Journal” J. Neurosci. 2006, 26 (39).

2002

Maeda T, Mazzulli JR, Farrance IK, Stewart AF. Mouse DTEF-1 (ETFR-1, TEF-5) is a transcriptional activator in alpha 1-adrenergic agonist-stimulated cardiac myocytes. J Biol Chem. 2002; 277: 24346-52. PMID: 11986313.

Book Chapters

Mazzulli JR, Hodara R, Lind S, Ischiropoulos H. Oxidative stress and protein deposition diseases. In Protein misfolding, aggregation and conformational diseases : part A, protein aggregation and conformational diseases. Uversky VN and Fink AL, editors. New York, N.Y.: Springer Science+Business Media. 2006; 123-136.

Mazzulli JR, Lind S, Ischiropoulos H. Reactive oxygen and nitrogen species: Weapons of neuronal destruction. In Oxidative stress and age-related neurodegeneration. Luo Y and Packer L, editors. Boca Raton: CRC/Taylor & Francis. 2006; 107-120.