Protocols

Download our lab protocols below.

Culturing Nerve Cells

• Preparation of Astroglia Cell Culture
• Preparation of Dissociated Hippocampal Culture

Determination of Cell Viability

• Fluorescence Cell LIVE/DEAD
• Trypan Blue Viability Assay - Attached Cells
• Trypan Blue Viability Assay - Cells in Suspension
• TUNEL Assay Staining for Cultured Cells on Coverslips
• TUNEL Assay Staining for Fixed Sections

lmmunocytochemistry

• lmmunocytochemistry - Cultured Cells
• lmmunohistochemistry (Brain Sections)

lmmunoprecipitation

• lmmunoprecipitation Protocol for Tissue Samples

Molecular Biology

• Generation of Plasmid cDNA
• REDextract Genotyping
• RNA Extraction from Cell Culture
• RNA Extraction from Tissue
• RT - PCR

Protease Activity Assays

• In Vitro Protease Activity Assays

Protein Aggregation

• Aggregating Beta Amyloid
• Aggregation of Tau
• Aggregation of Tau as evaluated by ThS Fluorescence
• Labelling Protein Aggregates
• Preparation of NFTs from AD Brain Tissue

Subcellular Fractioning

• Biotinylation and Precipitation of Cell Surface Proteins
• Cytoskeletal Fractioning
• Cytoskeletal Fractioning of Tissue
• F/G Actin Binding Assay
• Membrane/Organelle Fractioning
• Mitochondria Labelling - MitoTracker™ Protocol
• Obtaining Membrane-rich Protein Fractions
• Sarkosyl Fractionation Assay - Cell or Recombinant Protein
• Sarkosyl Fractionation Assay - Human Tissue
• Subcellular Fractionation
• Synaptosomal Preparations

Transfection of Neural Cells

• Calcium Phosphate Transfection Method
• Nucleofection of Dissociated Hippocampal Neurons in Suspension

Western Blot Analysis

• Native PAGE
• Western Blotting Protocol