Publications
In vitro and in vivo approaches to study kinetochore-microtubule attachments during mitosis
Afreen S, Rahi A, Chakraborty M, Landeros AG, Varma D.
Methods Molecular Biology. 2021 Paper Accepted.
Kinetochore-microtubule coupling mechanisms mediated by the Ska1 complex and Cdt1
Rahi A, Chakraborty M, Vosberg K, Varma D.
Essays Biochem. 2020 Sep 4;64(2):337-347. doi: 10.1042/EBC20190075.
Computational model demonstrates that Ndc80-associated proteins strengthen kinetochore-microtubule attachments in metaphase.
Campbell S, Amin MA, Varma D, Bidone TC.
Cytoskeleton (Hoboken). 2019 Nov;76(11-12):549-561. doi: 10.1002/cm.21562. Epub 2019 Oct 11.
Mapping the kinetochore MAP functions required for stabilizing microtubule attachments to chromosomes during metaphase.
Amin MA, Agarwal S, Varma D.
Cytoskeleton (Hoboken). 2019 Aug 27. doi: 10.1002/cm.21559.
Cell Division: The Unattached Kinetochore Wears an Expansive RZZ Coat.
Suzuki A and Varma D.
Curr Biol. 2018 Nov 5;28(21):R1250-R1252. doi: 10.1016/j.cub.2018.10.001.
Cdt1 stabilizes kinetochore-microtubule attachments via an Aurora B kinase-dependent mechanism.
Agarwal S, Smith KP, Zhou Y, Suzuki A, McKenney RJ and Varma D.
J Cell Biol. 2018 Aug 28. pii: jcb.201705127. doi: 10.1083/jcb.201705127
Antagonism between the dynein and Ndc80 complexes at kinetochores controls the stability of kinetochore-microtubule attachments during mitosis.
Amin MA, McKenney RJ, Varma D.
J Biol. Chem. 2018 Feb 23. pii: jbc.RA117.001699. doi: 10.1074/jbc.RA117.001699.
Combining mitotic cell synchronization with high-resolution confocal microscopy to study the role of multi-functional cell cycle proteins during mitosis.
Amin MA and Varma D.
J Vis. Exp., 2017 (in press), e56513, doi:10.3791/56513.
Targeting mitotic pathways for endocrine-related cancer therapeutics.
Agarwal S and Varma D.
Endocrine-related Cancer. 2017, June, 24(9), T65-T82
PMID: 28615236
The RZZ complex requires the N-terminus of KNL1 to mediate optimal Mad1 kinetochore localization in human cells.
Caldas GV, Lynch TR, Anderson R, Afreen S, Varma D*, DeLuca JG*.
Open Biol. 2015 Nov;5(11). pii: 150160.
PMID:26581576
How the SAC gets the axe: Integrating kinetochore microtubule attachments with spindle assembly checkpoint signaling.
Agarwal S, Varma D.
Bioarchitecture. 2015 Oct 2:1-12. [Epub ahead of print]
PMID:26430805
Sequential replication-coupled destruction at G1/S ensures genome stability.
Coleman KE, Grant GD, Haggerty RA, Brantley K, Shibata E, Workman BD, Dutta A, Varma D, Purvis JE, Cook JG.
Genes Dev. 2015 Aug 15;29(16):1734-46. doi:10.1101/gad.263731.115. Epub 2015 Aug 13.
PMID:26272819
Cell division: Molecular pathways for KMN kinetochore recruitment.
Afreen S, Varma D.
Curr Biol. 2015 Apr 20;25(8):R332-5. doi: 10.1016/j.cub.2015.02.041.
PMID: 25898103
CDK1-dependent inhibition of the E3 ubiquitin ligase CRL4CDT2 ensures robust transition from S phase to mitosis
Rizzardi LF, Coleman KE, Varma D, Matson JP, Oh S, Cook JG.
Journal of Biological Chemistry. 2015 Jan 2;290(1):556-567.doi:10.1074/jbc.M114.614701.
ISSN: 00219258
Spindle assembly checkpoint proteins are positioned close to core microtubule attachment sites at kinetochores
Varma D, Wan X, Cheerambathur D, Gassmann R, Suzuki A, Lawrimore J, Desai A, Salmon ED.
Journal of Cell Biology. 2013 Jan;202(5):735-746.doi:10.1083/jcb.201304197.
PMID: 23979716 ISSN: 00219525
The KMN protein network - Chief conductors of the kinetochore orchestra
Varma D, Salmon ED.
Journal of Cell Science. 2012 Dec 15;125(24):5927-5936.doi:10.1242/jcs.093724.
PMID: 23418356 ISSN: 00219533
Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore-microtubule attachment
Varma D, Chandrasekaran S, Sundin LJR, Reidy KT, Wan X, Chasse DAD, Nevis KR, Deluca JG, Salmon ED, Cook JG.
Nature Cell Biology. 2012 Jun;14(6):593-603.doi:10.1038/ncb2489.
PMID: 22581055 ISSN: 14657392
Removal of Spindly from microtubule-attached kinetochores controls spindle checkpoint silencing in human cells
Gassmann R, Holland AJ, Varma D, Wan X, Çivril F, Cleveland DW, Oegema K, Salmon ED, Desai A.
Genes and Development. 2010 May;24(9):957-971.doi:10.1101/gad.1886810.
PMID: 20439434 ISSN: 08909369
Development and application of in vivo molecular traps reveals that dynein light chain occupancy differentially affects dynein-mediated processes
Varma D, Dawn A, Ghosh-Roy A, Weil SJ, Ori-McKenney KM, Zhao Y, Keen J, Vallee RB, Williams JC.
Proceedings of the National Academy of Sciences of the United States of America. 2010 Feb 23;107(8):3493-3498.doi:10.1073/pnas.0908959107.
PMID: 20133681 ISSN: 00278424
Emerging functions of force-producing kinetochore motors
Mao Y, Varma D, Vallee R.
Cell Cycle. 2010 Feb 15;9(4):715-719.
PMID: 20160491 ISSN: 15384101
Direct role of dynein motor in stable kinetochore-microtubule attachment, orientation, and alignment
Varma D, Monzo P, Stehman SA, Vallee RB.
Journal of Cell Biology. 2008 Sep 22;182(6):1045-1054.doi:10.1083/jcb.200710106.
PMID: 18809721 ISSN: 00219525
ZW10 function in mitotic checkpoint control, dynein targeting and membrane trafficking: Is dynein the unifying theme?
Vallee RB, Varma D, Dujardin DL.
Cell Cycle. 2006 Nov;5(21):2447-2451.
PMID: 17102640 ISSN: 15384101
Role of the kinetochore/cell cycle checkpoint protein ZW10 in interphase cytoplasmic dynein function
Varma D, Dujardin DL, Stehman SA, Vallee RB.
Journal of Cell Biology. 2006 Feb 27;172(5):655-662.doi:10.1083/jcb.200510120.
PMID: 16505164
Dynein: An ancient motor protein involved in multiple modes of transport
Vallee RB, Williams JC, Varma D, Barnhart LE.
Journal of Neurobiology. 2004 Feb 5;58(2):189-200.doi:10.1002/neu.10314.
PMID: 14704951