Uncovering the molecular mechanisms of GPCR signaling.
The Ziarek Lab seeks to define the molecular mechanisms underlying G protein-coupled receptor (GCPR) allostery and functional selectivity. By integrating biophysical, biochemical and cellular approaches, we aim to uncover how distinct receptor conformations drive specific signaling responses, with the long-term goal of enabling more precise and effective therapeutic strategies.
Featured Research
Bower et al. 2026, Protein Science
Stabilization versus flexibility: Detergent-dependent trade-offs in neurotensin receptor 1 GPCR ensembles
Shriver et al. 2026, Protein Science
Intrinsic conformational equilibria position arrestin-2 for activation.
Bower et al. 2024, Protein Science
Insights on the G protein-coupled receptor helix 8 solution structure and orientation using a neurotensin receptor 1 peptide.
Bumbak et al. 2023, Nature Communications
β-arrestin-1 and arrestin-biased ligands slow the rate of conformational exchange
Bumbak et al. 2023, Nature Communications
Orthosteric ligands and lipid cofactors remodel the NTS1 kinetic and thermodynamic ensemble
Dixon et al. 2022, JACS
Effect of ligands and transducers on the Neurotensin receptor 1 conformational ensemble
Bumbak et al. 2022, Cell Reports
Density-functional theory (DFT) calculations lend confidence to structural interpretations
Dixon et al. 2022, Protein Science
Selective 19 F-labeling absent of probe sequestration (SLAPS) protocol
Bumbak et al. 2022, Cell Reports
The amplitude of sub-microsecond motion correlate with ligand pharmacology
Robson et al. 2021, J Biomol NMR
TRACT revisited: an algebra solution for determining overall rotational correlation times from cross-correlated relaxation rates
