Microbes are critical for the health of humans and animals. The microbial consituents of animals—their microbiomes—are often newly acquired during each host generation. Our lab asks how beneficial bacteria are reproducibly acquired from the environment by animal hosts. We focus on the natural Vibrio-squid system to understand the processes that underlie colonization. Both organisms may be obtained directly by environmental collection, cultured independently, and then reintroduced in the laboratory to discover and interrogate molecular communication. Studying this powerful model we are investigating signal transduction within and between colonizing partners.
Brooks JF, Mandel MJ. 2016. The histidine kinase BinK is a negative regulator of biofilm formation and squid colonization. J Bacteriol doi:10.1128/JB.00037-16
Brooks JF, Gyllborg MC, Cronin DC, Quillin SJ, Mallama CA, Foxall R, Whistler CA, Goodman AL, Mandel MJ. 2014. Global discovery of colonization determinants in the squid symbiont Vibrio fischeri. Proc Natl Acad Sci USA 111:17284–17289.
Wang N, Ozer EA, Mandel MJ, Hauser AR. 2014. Genome-wide identification of Acinetobacter baumannii genes necessary for persistence in the lung. mBio 5:e01163-14.