Research
Research in our laboratory is focused on the following areas:
Biology of the Aging Lung
We seek to understand why regeneration of the alveolus, the gas-exchanging part of the lung, fails in older people and how that is linked to ongoing inflammation.
Molecular Pathology
Pathology is the underpinning of medicine. We seek to understand pneumonia at the level of the single cell by applying emerging technologies (single cell RNA sequencing and spatial transcriptomics, proteomics, and metabolomics) to samples we collect as part of clinical care.
Data Science
We seeks to integrate clinical data with molecular pathology to identify disease endotypes likely to respond to therapies we identify in the laboratory.
Image Gallery
Combined immunofluorescence and smFISH (RNAScope™) from postmortem human frontal lobe tissue. Blue, DAPI; green, IBA1 (microglia); red, IL1B; cyan, CCL2; magenta, CDKN1A.
From Grant et al. 2023, Prolonged exposure to lung-derived cytokines is associated with inflammatory activation of microglia in patients with COVID-19, bioRxiv 2023.07.28.550765 (preprint).
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Combined immunofluorescence and smFISH (RNAScope™) from postmortem human frontal lobe tissue. Blue, DAPI; green, IBA1 (microglia); red, IL1B; cyan, CCL2; magenta, CDKN1A.
From Grant et al. 2023, Prolonged exposure to lung-derived cytokines is associated with inflammatory activation of microglia in patients with COVID-19, bioRxiv 2023.07.28.550765 (preprint).
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Alveolospheres in culture.
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Fluorescent imaging of alveolospheres in culture. Green, alveolar type 2 (AT2) epithelial cells; pink, alveolar type 1 (AT1) epithelial cells; blue, DAPI.
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Muscle cells. Green, muscle fibers; red, macrophages; blue, DAPI.
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Muscle cells. Green, muscle fibers; red, macrophages; blue, DAPI.
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